Pets at Home is investing more than £444,000 in a new store at the Mid Sussex Retail Park, Burgess Hill, which opens on August 23.
The move will create eight new jobs for the local area.
Store manager Simon Bailey said: “I'm delighted to be opening our new store in Burgess Hill.
“We are all passionate about pets, which is evident to everyone who visits a Pets at Home store. Every day brings new challenges and it’s never the same two days running. I enjoy interacting with customers and giving them the help and advice they need.
“Thanks to Pets at Home I am fully qualified to advise on all types of pets from rabbits, guinea pigs and chinchillas to fish and reptiles.”